细胞凋亡检测试剂盒  640914  100 tests  biolegend

FITC Annexin V Apoptosis Detection Kit with PI

Reactivity:     All mammalian species
Storage & Handling:     Store between 2°C and 8°C. Do not freeze.

Caution: Propidium Iodide Solution is toxigenic and mutagenic; handle with care.
Application:     

FC - Quality tested
Recommended Usage:     

Staining Procedure:
1. Wash cells twice with cold BioLegend's Cell Staining Buffer, and then resuspend cells in Annexin V Binding Buffer at a concentration of 0.25-1.0 x 107 cells/ml.
2. Transfer 100 µl of cell suspension in a 5 ml test tube.
3. Add 5 µl of FITC Annexin V.
4. Add 10 µl of Propidium Iodide Solution.
5. Gently vortex the cells and incubate for 15 min at room temperature (25°C) in the dark.
6. Add 400 µl of Annexin V Binding Buffer to each tube. Analyze by flow cytometry with proper machine settings.
Application
Notes:     

Materials Provided:
0.5 ml of FITC Annexin V
1 ml of Propidium Iodide Solution
50 ml of Annexin V Binding Buffer

Materials Not Included:
Cell Staining Buffer (Cat. No. 420201)
Application
References:     

1. Ranhanathan P, et al. 2013. J AM Soc Nephrol. PubMed

货号                      产品名称                       规格                        促销价

640914 FITC Annexin V Apoptosis Detection Kit with PI 100 tests  2398
640922FITC Annexin V Apoptosis Detection Kit with 7-AAD100 tests 2398

640930 APC Annexin V Apoptosis Detection Kit with 7-AAD 100 tests 2463
640932APC Annexin V Apoptosis Detection Kit with PI100 tests         2463

420403/420404 7-AAD Viability Staining Solution 200tests/500tests 363/625
421301Propidium Iodide Solution2 ml（200tests）493

422201 Annexin V Binding Buffer 100 ml 295
640905/640906FITC Annexin V25 tests/100 tests  855/2073

640907/640908 PE Annexin V 25 tests/100 tests 1020/2203
640919/640920APC Annexin V25 tests/100 tests 1053/2203

640911/640912 Alexa Fluor® 647 Annexin V 25 tests/100 tests 1053/2300