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您现在的位置:首页 > 产品中心 > 蛋白L,FITC-Labeled Recombinant Protein L

蛋白L,FITC-Labeled Recombinant Protein L

货  号:RPL-PF141

产品规格:200ug

原  产  地:

参考价格:1200 (参考价格,以实际价格为准)

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产品详细信息

FITC-Labeled Recombinant Protein L

蛋白L,FITC-Labeled Recombinant Protein L

分子别名(Synonym)

RPL,Protein L

表达区间及表达系统(Source)

FITC-Labeled Recombinant Protein L (Cat. No. RPL-PF141) is expressed from human HEK293 cells. It is the FITC labeled form of Recombinant Protein L (Cat. No. RPL-P3141).


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蛋白结构(Molecular Characterization)

Online()

FITC-Labeled Recombinant Protein L is fused with a polyhistidine tag at the N-terminus. The reducing (R) protein migrates as 40-45 kDa in SDS-PAGE.

Conjugate

FITC

Excitation source: 488 nm spectral line, argon-ion laser

Excitation Wavelength: 488 nm

Emission Wavelength: 535 nm

标记(Labeling)

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular seive treatment during purification process.

蛋白标记度(FITC:Protein Ratio)

The FITC to protein molar ratio is 5-7.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Yervoy Ipilumimab at 10 μg/mL (100 μL/well) can bind FITC-Labeled Recombinant Protein L (Cat. No. RPL-PF141) with a linear range of 20-156 ng/mL (Ex.488 nm/Em.535 nm) (QC tested).


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