Human CD19 (20-291) Protein, His Tag

分子别名（Synonym）

CD19,B4,CVID3,MGC12802

表达区间及表达系统（Source）

Human CD19 (20-291), His Tag (CD9-H52H2) is expressed from human 293 cells (HEK293). It contains AA Pro 20 - Lys 291 (Accession # P15391-1).

Predicted N-terminus: Pro 20

Request for sequence

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 32.0 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by reduced SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

电泳（SDS-PAGE）

Human CD19 (20-291), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

活性（Bioactivity）-ELISA

Immobilized FMC63 MAb at 2 μg/mL (100 μL/well) can bind Human CD19 (20-291), His Tag (Cat. No. CD9-H52H2) with a linear range of 10-78 ng/mL (QC tested).

Protocol

Immobilized Human CD19 (20-291), His Tag (Cat. No. CD9-H52H2) at 2 μg/mL (100 μL/well) on Nickel Coated Plate, can bind FMC63 (Mouse IgG2a) with a linear range of 2-13 ng/mL (Routinely tested).

Immobilized Human CD19 (20-291), His Tag (Cat. No. CD9-H52H2) at 0.1 μg/mL (100 μL/well) on MonRabTM Anti-His Tag (C-term) Antibody precoated (0.1 μg/well) plate, can bind FMC63 (Mouse IgG2a) with a linear range of 0.6-10 ng/mL (Routinely tested).

活性（Bioactivity）-SPR

FMC63 MAb (mouse lgG2a) captured on CM5 chip via anti-mouse antibodies surface, can bind Human CD19 (20-291), His Tag (Cat. No. CD9-H52H2) with an affinity constant of 2.95 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

CAR阳性表达率检测（Evaluation of CAR expression ）

FACS Analysis of anti-CD19 CAR Expression

293 cells were transfected with FMC63-scFv and RFP tag. 2x105 of the cells were first incubated with A. His Tag-protein control. B. Recombinant human CD19 (20-291), His Tag (Cat. No. CD9-H52H2, 10 μg/ml). C. Recombinant human CD19, His Tag (Cat. No. CD9-H52H2, 10 μg/ml) and FMC63 (Mouse anti-CD19 antibody). The FITC Anti-6xHis tag antibody was used to analyse with FACS. RFP was used to evaluate CAR (FMC63-scFv) expression and FITC was used to evaluate the binding activity of recombinant human CD19 (20-291), His Tag (Cat. No. CD9-H52H2).