Protein Transport Inhibitor Cocktail (500X)

00-4980-03

4*100

USA

The Cell Stimulation Cocktail (plus protein transport inhibitors) (500X)is a

cocktail of phorbol 12-myristate 13-acetate (PMA), ionomycin, brefeldinA and
monensin. Treatment with PMA and ionomycin is sufficient to induce activation
of many cell types to produce cytokines. Brefeldin A and Monensinresult in the
accumulation of secreted proteins in the endoplasmic reticulum andGolgi apparatus.
Thus, the Cell Stimulation Cocktail (plus protein transport inhibitors)(500X) can
be used for induction and subsequent intracellular detection ofcytokines and other
secreted proteins in both in vitro-cultured and ex vivo cells.
 
For an unstimulated and vehicle control, see the Protein Transport
Inhibitor Cocktail (500X) (cat. 00-4980).