APC Annexin V Apoptosis Detection Kit with PI 640932  100T

Reactivity:     All mammalian species
Storage & Handling:     Store between 2°C and 8°C. Do not freeze.

Caution: Propidium Iodide Solution is toxigenic and mutagenic; handle with care.
Recommended Usage:     

Staining Procedure:
1. Wash cells twice with cold BioLegend's Cell Staining Buffer, and then resuspend cells in Annexin V Binding Buffer at a concentration of 0.25-1.0 x 107 cells/ml.
2. Transfer 100 µl of cell suspension in a 5 ml test tube.
3. Add 5 µl of FITC Annexin V.
4. Add 10 µl of Propidium Iodide Solution.
5. Gently vortex the cells and incubate for 15 min at room temperature (25°C) in the dark.
6. Add 400 µl of Annexin V Binding Buffer to each tube. Analyze by flow cytometry with proper machine settings.
Application
Notes:     

Materials Provided:
0.5 ml of FITC Annexin V
1 ml of Propidium Iodide Solution
50 ml of Annexin V Binding Buffer

Materials Not Included:
Cell Staining Buffer (Cat. No. 420201)

pplication
References:

1. Ranhanathan P, et al. 2013. J AM Soc Nephrol. PubMed